Skip to content

Interaction-seq

Computational Method

I. RNA-RNA

  • 2025 Nature communications biology - Cellular dsRNA interactome captured by K1 antibody reveals the regulatory map of exogenous RNA sensing

  • [review] 2024 Molecular Cell - Technological advancements in deciphering RNA-RNA interactions

  • 2024 Cell - snoRNA-facilitated protein secretion revealed by transcriptome-wide snoRNA target identification

  • 2024 Nature Biotechnology - KARR-seq reveals cellular higher-order RNA structures and RNA–RNA interactions

  • 2023 Nature - Complementary Alu sequences mediate enhancer–promoter selectivity

  • [review] 2022 Current Opinion in Genetics & Development - Architecture of RNA–RNA interactions

  • 2020 Nature - RIC-seq for global in situ profiling of RNA–RNA spatial interactions

  • 2020 Nature - RIC-seq for global in situ profiling of RNA–RNA spatial interactions.

II. RNA-DNA+

  • MUSIC-seq: 2024 Nature - Single-cell multiplex chromatin and RNA interactions in ageing human brain
  • 2024 Nature Leukemia - The landscape of RNA-chromatin interaction reveals samll non-coding RNAs as essential mediators of leukemia maintenance
  • 2022 Nature Protocols - SPRITE: a genome-wide method for mapping higher-order 3D interactions in the nucleus using combinatorial split-and-pool barcoding
  • 2017 Nature Biotech. - GRID-seq reveals the global RNA–chromatin interactome

III. Off-target

Computational Methods

1. Screen methods

  • 2024 Nature Biotechnology - Pooled CRISPR screens with joint single-nucleus chromatin accessibility and transcriptome profiling

2. sgRNA offtarget - experimental methods

  • 2024 Nat. Biotech. - Tracking-seq reveals the heterogeneity of off-target effects in CRISPR–Cas9-mediated genome editing
  • 2023 Nat. Methods - Improving the sensitivity of in vivo CRISPR off-target detection with DISCOVER-Seq+
  • 2019 Science - Unbiased detection of CRISPR off-targets in vivo using DISCOVER-Seq